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KMID : 0380020090240020212
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 2 p.212 ~ p.215
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Optimization of liquid-liquid extraction conditions for paclitaxel separation from plant cell cultures
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Kim Jin-Hyun
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Abstract
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In this study, the process parameters of liquid-liquid extraction were optimized to obtain a high purity and yield of paclitaxel in a pre-purification step. The optimal solvent ratio (methylene chloride/concentrated methanol extract ratio), extraction times, mixing time, and standing time for liquid-liquid extraction were 0.28 (v/v), 3(times), 30 min, and 40 min, respectively. The polar impurities from the biomass extraction were efficiently removed by liquid-liquid extraction. The complete concentration of liquid-liquid extract by rotary evaporator was reliable enough to obtain a high purity and yield of paclitaxel for subsequent purification steps.
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KEYWORD
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paclitaxel, separation, liquid-liquid extraction, process parameters, optimization
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